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. 2019 Dec;101:103449. doi: 10.1016/j.dci.2019.103449

Fig. 1.

Fig. 1

Western blot detection of recombinant (A) and endogenous rainbow trout IL-22 (B). (A) Recombinant IL-22 (lane 1, 100 ng) and rIL-2B (lane 2, 100 ng, Wang et al., 2018) proteins were fractionated in a 4–12% (w/v) Bis-Tris SDS-PAGE under reducing conditions, stained with SimplyBlue™ SafeStain (left) and immunostained with anti-trout IL-22 mAb L7 or L8 (right). MW = Magic-Marker™ XP Western Protein standard (Invitrogen). (B) Cell lysates from head kidney leucocytes cultured without (lane 1) or with rIL-21 (lane 2) were fractionated in a 4–12% (w/v) Bis-Tris SDS-PAGE under reducing conditions, stained with SimplyBlue (left) and immunostained with anti-trout IL-22 mAb L7 or L8 (right).