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. 2019 Dec;101:103449. doi: 10.1016/j.dci.2019.103449

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© 2019 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 5 — IL-22-producing cells in gill leucocytes after Y. ruckeri infection. Rainbow trout were infected by i. p. injection of Y. ruckeri (ERM) or PBS as control. Gill samples were collected at 24 h post challenge (hpc) and leucocytes prepared. Gill leucocytes were immunostained with anti-IL-22 mAb L8 and analysed by flow cytometry. Representative results are shown in (A) Gating of the leucocyte population and (B) Gating of IL-22⁺ cells. (C) The percentage of IL-22⁺ cells in total leucocytes (mean +SE, N = 6). * indicates a significant difference between control and infected fish (p ≤ 0.05, independent samples T-test).