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. 2019 Nov 15;10:1215. doi: 10.3389/fneur.2019.01215

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Copyright © 2019 Jing, Yang, Fu, Wang, Yang, Yuan, Chen, Ding, Chen and Tian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Serpina3k reduced damage to SH-SY5Y cells after SI. (A) Cell viability 4 h after treatment with different concentrations of serpina3k as determined using a CCK-8 assay. (B) Effect of different concentrations of serpina3k on the cytotoxicity of SH-SY5Y cells at 4 h after SI, as evaluated based on LDH release. Detection of apoptotic cells using Annexin V-FITC/PI double staining with flow cytometry analysis (C) and TUNEL staining (E). Scale bar = 30 μm. Quantification of apoptosis rate (FITC+, D; TUNEL+, F) at 4 h after SI in the control, SI+vehicle, and SI+A3K groups. Data represent the mean ± SD (n = 5 per group). *P <0.05, **P <0.01; ^#P > 0.05.