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. 2019 Nov 15;9:1189. doi: 10.3389/fonc.2019.01189

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Copyright © 2019 Lu, Xie, Wang, Zheng, Zhang, Deng, Shi, Zhong, Chen, Zhang, Xu, Shammas and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interactions between MUS81 and proteins involved in cell cycle progression. (A) The workflow of the Cell Cycle AntibodyArray™. (B) The results of the Cell Cycle AntibodyArray™ assay. Proteins captured on the array were detected by immunoblotting (the positions of 1–5 are CyclinB, BM28, BRCA1, BRCA2, and Nibrin proteins, respectively), and the number of target proteins was calculated by gray scanning. (C) The relationships between MUS81 and other proteins were confirmed by western blot or qPCR. (I) The levels of MUS81 and BRCA1, BRCA2 mRNA were assayed by qPCR. (II) The levels of MUS81 and BM28 protein expression were analyzed by western blot. Data are presented as the mean ± SD of three independent experiments (^*P < 0.05, ^**P < 0.01). (D) The potential interaction of MUS81 and BM28 was analyzed by Discover Studio.