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. 2019 Nov 15;7:321. doi: 10.3389/fbioe.2019.00321

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Copyright © 2019 Wang, Fu, Yan, Zhang and Pei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Adipogenic potential of human IPFSCs after FN1-KO. Human IPFSCs were adipogenically induced in differentiation medium for 21 days. The effect of FN1-KO on adipogenic capacity of human IPFSCs was evaluated using Oil Red O staining for liquid droplets (A) and qPCR for adipogenic marker gene (LPL, PPARG, FABP4, and CEBPA) expression (B). GAPDH was used as an endogenous control. Data are shown as bar charts. *indicates a significant difference compared to the corresponding copGFP group (P < 0.05).