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. 2019 Nov 15;10:1341. doi: 10.3389/fphar.2019.01341

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Copyright © 2019 Zhu, Tang, Zhang, Wei, Yang, He, Zheng and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in ROS levels induced by a14 were probed by DCFH-DA in HepG2 cells. (A) Determination of fluorescence intensity in HepG2 cells under different conditions by flow cytometry; (B) Fluorescence intensity comparison chart with or without NAC; (C) Quantitative analysis of fluorescence intensity in HepG2 cells under different conditions; (D) Quantitative analysis of fluorescence intensity with or without NAC blockers; (E) Flow Cytometric analysis was applied to evaluate the pro-apoptosis of a14 (50 µM) on HepG2 cells for 48 h with or without the intervention of NAC (5 mM). Values are expressed as the means ± SE, by t test, n = 3, **P < 0.01, ***P< 0.001 vs. the black group (cell + DCF).