Figure 2.

Exposure to visible light significantly increases the transcription of sigB, lmo2230, and opuCA compared to the dark control at 30°C but not 37°C. Cells were grown to OD ~ 0.2 at 30 or 37°C and exposed to 0.6 mW cm⁻² 470 nm light for 30 min at the same temperature, with RNA samples collected at 10 min intervals. Relative transcription of sigB (A), lmo2230 (B), and opuCA (C) was measured by RT-PCR. Error bars represent SD from two independent replicates. Statistical significance was determined by a paired t-test. (^*p ≤ 0.05; ^****p ≤ 0.0001).