Figure 3.

The expression of lmo0799 is unaffected by temperature. (A) Cells were grown to OD ~ 0.2 at 30 or 37°C and exposed to 0.6 mW cm⁻² 470 nm light for 30 min at the same temperature, with RNA samples collected at 10 min intervals. Relative transcription of lmo0799 to T0 at each temperature was measured by RT-PCR. Error bars represent SD from two independent replicates. Statistical significance was determined by two-way ANOVA with Dunnett’s multiple comparisons test. (B) Protein extracts from stationary phase cultures of the wild-type, ΔsigB and Δlmo0799 strains grown at 30 or 37°C in darkness were standardized to 0.55 mg ml⁻¹ and separated via SDS-PAGE. The levels of Lmo0799 in cultures were determined by western blot with polyclonal anti-Lmo0799 antibodies, with the Δlmo0799 strain as a negative control. Image is representative of three independent replicates.