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. 2019 Nov 12;7:277. doi: 10.3389/fcell.2019.00277

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Copyright © 2019 Xue, Liu, Wen, Yang, Gao, Tao and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The establishment of a zebrafish vgll4b knockout line. (A) Schematic representation of Cas9 target site in the first exon of zebrafish vgll4b. Dr: Danio rerio. The deleted nucleotides in the mutant gene are marked by hyphens. (B) Schematic representation of wild type (282 amino acids) and mutant Vgll4b proteins (169 amino acids). The site where the frameshift was introduced is marked by triangles. (C) Western blot analysis of HA-tagged wild type and mutant Vgll4b proteins. NC: non-specific control. (D) Luciferase analysis of HEK293T cells transfected with TEAD1, YAP, VGLL4, and wild type or mutant Vgll4b expressing plasmids. Luciferase activity was normalized to empty vector pcDNA3.1 which was set to 1.0. Error bars represent ± SD of at least three replicates. p values are denoted by asterisks. ^∗∗∗P < 0.001 (ANOVA test).