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. 2019 Nov 13;10:2617. doi: 10.3389/fimmu.2019.02617

Figure 2.

Figure 2

Neutrophilic NETs induce the alarmin S100A9. Neutrophilic NETs were generated from H&N GPA, systemic GPA, and age-matched healthy individuals and added to CD14+ monocytes (5,000 ng/ml) for 24 h. Transcript expression was quantified by RT-PCR. (A) Transcripts for the alarmins S100A8 and S100A9 (n = 5, each group). (B,C) Intracellular expression of S100A9 protein measured by flow cytometry. Representative contour plots (B) and summary results from five experiments (C). (D–G) Transcript expression for IFNα, IFNβ, IFNγ, and IL-1β (n = 5, each group). All data are mean ± SEM. (A,C) One-way ANOVA with post-hoc Tukey's multiple comparisons test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns P > 0.05.