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S100A9 upregulates monocyte MMP production. Purified monocytes were treated with recombinant S100A9 (100 ng/ml) or vehicle for 24 h. Transcripts for MMP-2, MMP-3, and MMP-9 were quantified by RT-PCR (n = 6) (A). Intracellular MMP-9 protein expression was analyzed by flow cytometry (B,C). Representative histograms (B) and data from seven experiments (C). Paired t-test. *P < 0.05, **P < 0.01.
