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. 2019 Nov 13;10:2617. doi: 10.3389/fimmu.2019.02617

Figure 5.

Figure 5

NETs-induced S100A9 promotes MMP-9 production. NETs were induced from neutrophils of GPA patients and matched controls. Monocytes were treated with isolated NETs for 24 h. (A) Transcripts for MMPs were assessed by RT-PCR. (B,C) Flow cytometrical quantification of intracellular MMP-9 protein. Representative histograms (B) and MFIs from five experiments (C). (D,E) S100A9 receptor binding was blocked with paquinimod in NET-monocyte cocultures. MMP-9 protein was quantified by flow cytometry as in (B). Representative histograms (D) and results from six experiments (E). NETs from H&N GPA patients were treated with DNase for 15 min and added to monocytes for 24 h. Intracellular MMP-9 expression was examined using flow cytometry. Representative histogram (F) and MFIs from six experiments (G). All data are mean ± SEM. (A,C,E) One-way ANOVA with post-hoc Tukey's multiple comparisons test. (G) Paired t test. *P < 0.05; **P < 0.01; ***P < 0.001.