Figure 9.
Inflammatory cytokines determined in serum from tumor-bearing mice treated with the recombinant Salmonella strains. Tumor-bearing mice were treated with four doses of 1 × 107 colony-forming units (CFU) in the tail vein of the recombinant Salmonella strains and evaluated for the antitumor activity (tumor size) and were euthanized after 5 days of the last inoculation. Serum from those mice was analyzed for the presence of inflammatory cytokines as IL-6, IL-10, MCP-1, IFN-γ, TNF-α, and IL-12p70 using the Cytometric Bead Array (CBA) Mouse Inflammation Kit (BD Biosciences). Samples were acquired in the flow cytometry BD FACSCanto II, and the output was processed in the FCAP Array software v3.0. (BD Biosciences). The results are representative of three independent experiments. ANOVA test was performed with Bonferroni post hoc for the difference between the groups. For MCP-1, *p < 0.005, **p < 0.001, ***p < 0.0001; For IL-12, *p < 0.009, **p < 0.001; For IFN-γ, *p < 0.001, **p < 0.0002.