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. 2019 Sep 11;3(11):e10227. doi: 10.1002/jbm4.10227

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© 2019 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) Cre activity was induced by five consecutive doses of tamoxifen followed by a 3‐week clearance. Five‐month‐old mice had their right tibias loaded for 5 consecutive days. Mice received 5‐bromo‐2′‐deoxyuridine (BrdU) in their drinking water at the start of loading, calcein green on day 4, and were euthanized on day 5. (B) Longitudinal cryosections were cut to intersect the posterolateral surface of the tibia. (C, D) Representative images of the region of interest from nonloaded and loaded tibias. (E, F) 40× Magnifications from nonloaded and loaded tibias. (G) Total Ps.Osx⁺ (periosteum osterix positive) surface of nonloaded and loaded tibias. (H) Increase in Ps.Osx⁺ calcein⁺ surface with mechanical loading. (I) A trend for an increase was found in the number of periosteal Osx⁺ cells on the bone surface with loading. Data shown as mean ± SD (n = 9; males = 4, females = 5). * Signifies p < .05 and ~ signifies 0.05 < p < 0.1 by Student's t test. tdTomato = red; calcein = green; 4,6‐diamidino‐2‐phenylindole (DAPI) = blue; Mu = muscle; Ps = periosteum; B = bone.