Table 2.
Bone Mineralization Density Distribution of Trabecular and Cortical Bone From the Femur
| 6 Weeks | 4 Months | 18 Months | Two‐way ANOVA (p values) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| WT (n = 8 ) | Shn3–/– (n = 8) | WT (n = 6) | Shn3–/– (n = 6) | WT (n = 8) | Shn3–/– (n = 8) | inter‐action | geno‐type | Age | ||
| Trabecular Bone | CaMean | 22.30 | 22.63 | 22.41 | 25.24 a , d | 23.10 | 24.88 a , d | .004 | <.001 | <.001 |
| (wt% Ca) | (0.59) | (1.16) | (0.53) | (0.60) | (1.45) | (0.73) | ||||
| CaPeak | 23.40 | 23.18 | 24.52 | 25.79 c , d | 24.33 | 25.84 b , d | .019 | .003 | <.001 | |
| (wt% Ca) | (0.61) | (1.27) | (0.59) | (0.77) | (1.03) | (0.71) | ||||
| CaWidth | 3.12 | 3.25 | 3.76 e | 3.29 c | 3.49 | 3.57 | .035 | .395 | .005 | |
| (Δwt%Ca) | (0.13) | (0.43) | (0.53) | (0.16) | (0.25) | (0.21) | ||||
| CaLow | 9.38 | 8.81 | 11.43 | 3.52 a , d | 7.98 h | 4.04 a , d | <.001 | <.001 | <.001 | |
| (% B.Ar) | (1.62) | (2.69) | (2.23) | (0.35) | (3.15) | (1.13) | ||||
| Cortical Bone | CaMean | 23.94 | 23.99 | 25.55 e | 25.88 d | 26.27 d | 27.08 d , h | .420 | .121 | <.001 |
| (wt% Ca) | (1.04) | (1.14) | (0.69) | (0.75) | (0.43) | (0.55) | ||||
| CaPeak | 24.87 | 24.57 | 26.23 e | 26.51 d | 26.82 d | 27.49 d | .215 | .359 | <.001 | |
| (wt% Ca) | (0.86) | (1.08) | (0.77) | (0.75) | (0.43) | (0.59) | ||||
| CaWidth | 3.01 | 3.01 | 2.92 | 3.18 c | 3.03 | 3.70 a , d , g | <.001 | <.001 | <.001 | |
| (Δwt%Ca) | (0.16) | (0.13) | (0.53) | (0.14) | (0.13) | (0.38) | ||||
| CaLow | 4.19 | 3.69 | 2.54 f | 2.31 f | 1.76 d | 1.51 d | .814 | .430 | <.001 | |
| (% B.Ar) | (1.92) | (1.07) | (2.23) | (0.33) | (0.44) | (0.42) | ||||
Data show mean (SD). n = number of studied animals. In bold: data that are significantly different to WT at similar age. Significance by Tukey post hoc comparison following two‐way ANOVA is indicated by superscript letters.
CaMean = weighted mean calcium concentration; CaPeak = most frequent calcium concentration; CaWidth = full width at half maximum of the bone mineralization density distribution, an index for the heterogeneity of mineralization; CaLow = percentage of bone area having a calcium concentration below 17.68 wt% Ca, representing the percentage of low mineralized lamellar bone undergoing the primary bone mineralization process; B.Ar = Bone Area.
p < .001.
p < .01.
p < .05 versus WT.
p < .001.
p < .01.
p < .05 versus 6 weeks.
p < .001.
p < .05 versus 4 months (all based on Tukey post hoc comparison).