Table 4.
Osteocyte Lacunae Sections (OLS) Characteristics of Cortical Bone of the Femur
| 6 Weeks | 4 Months | 18 Months | Two‐way ANOVA (p values) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| WT (n = 8 ) | Shn3–/– (n = 8) | WT (n = 6) | Shn3–/– (n = 6) | WT (n = 8) | Shn3–/– (n = 8) | Inter action | geno‐ type | Age | |
| OLS‐porosity (% ) | 1.4 | 1.7 c | 0.9 e | 1.2 c , e | 0.7 d | 0.9 d | ns | <.001 | <.001 |
| (0.4) | (0.4) | (0.1) | (0.1) | (0.1) | (0.1) | ||||
| OLS‐density (nb./mm2) | 603 | 629 | 467 d | 519 e | 474 d | 509 e | ns | ns | <.001 |
| (112) | (79) | 25 | 16 | (45) | 29 | ||||
| OLS‐area (μm2) | 23.8 | 27.7 a | 19.5 e | 23.9 b , e | 15.6 d , g | 18.8 b, d , f | ns | <.001 | <.001 |
| (2.3) | (3.6) | (0.9) | (1.4) | (2.1) | (1.8) | ||||
| OLS‐perimeter (μm) | 21.3 | 22.1 | 19.5 e | 21.0 b | 17.6 d , g | 19.0 b , d , g | ns | <.001 | <.001 |
| (0.8) | (1.5) | (0.3) | (0.8) | (1.2) | (1.0) | ||||
Data are mean (SD). n = number of studied mice. In bold: data that are significantly different to WT at similar age (based on post hoc comparison). Significance by Tukey post hoc comparison following two‐way ANOVA is indicated by superscript letters.
a
p = .001.
b
p < .01.
c
p < .05 versus WT.
d
p ≤ .001.
e
p < .01.
f
p < .001.
g
p < .01 18 months versus 4 months (all based on post hoc comparison).