Ross River virus (RRV) notifications by week, Brisbane local government area, Queensland, Australia, July 1, 2011–June 30, 2015 (A), and July 1, 2014–June 30, 2015 (B). Symbols in panel B represent single detection events: red triangles, RRV RNA detection from Flinders Technology Associates cards by real-time reverse transcription PCR; blue diamonds, RRV RNA detection from mosquito pools by real-time reverse transcription PCR; and black square, RRV detection from mosquito pools by cell culture ELISA. Also in panel B, the black line above the graph indicates when Flinders Technology Associates cards were deployed and gray line when mosquito pools were being collected and screened for RRV infection. Mosquitoes acquired from traps in weeks 10 and 11 were damaged by rain; thus, RRV-positive mosquito parts might have stuck to RRV-negative mosquitoes and turned some pools artificially positive.