Table 1. Proteins identified by mass spectrometry.
| Nº | Status | Gel Lane | Protein Identified | NCBI | % Cov† | Score | Peptides | Mass (Da) |
|---|---|---|---|---|---|---|---|---|
| 1 | Pre | 1 | Cystatin S precursor | NP_001890 | 12 | 89 | 1 | 16489 |
| 2 | Pre | 1 | Cystatin SN–partial peptide, 35 aa | AAB20561 | 51 | 73 | 1 | 4131 |
| 3 | Post | 2 | Cystatin SN precursor | NP_001889 | 12 | 69 | 1 | 16605 |
| 4 | Post | 2 | Cystatin S acidic isoform–partial peptide, 35 aa | AAB20560 | 51 | 57 | 1 | 4022 |
| 5 | Post | 2 | Cystatin SA-III | AAB19889 | 80 | 275 | 8 | 14409 |
| 6 | Pre | 3 | Keratin 1 | AFA52006 | 32 | 583 | 16 | 66197 |
| 7 | Pre | 3 | Keratin, type I cytoskeletal 9 | NP_000217 | 24 | 419 | 10 | 62255 |
| 8 | Post | 4 | Cystatin-SN precursor | NP_001889 | 60 | 473 | 10 | 16605 |
| 9 | Post | 4 | Cystatin-SA precursor | NP_001313 | 34 | 117 | 3 | 16719 |
| 10 | Post | 4 | Unnamed protein product | BAG36698 | 34 | 682 | 17 | 66151 |
| 11 | Post | 4 | Cystatin S acidic isoform–pkakanisartial peptide, 35 aa | AAB20560 | 51 | 57 | 1 | 4022 |
| 12 | Pre | 5 | Cystatin S precursor | NP_001890 | 18 | 139 | 2 | 16489 |
| 13 | Pre | 5 | Keratin, type II cytoskeletal 2 epidermal | NP_000414 | 17 | 105 | 5 | 65678 |
| 14 | Pre | 5 | Keratin, type I cytoskeletal 10 | NP_000412 | 15 | 205 | 6 | 58994 |
| 15 | Pre | 5 | Cystatin SN–partial peptide, 35 aa | AAB20561 | 51 | 57 | 1 | 4131 |
| 16 | Pre | 5 | Thioredoxin isoform 1 | NP_003320 | 12 | 27 | 1 | 12015 |
| 17 | Pre | 5 | Alpha-amylase 1 precursor | NP_004029 | 8 | 60 | 2 | 58415 |
| 18 | Post | 6 | Cystatin B | NP_000091 | 12 | 31 | 1 | 11190 |
| 19 | Post | 6 | Cystatin D | CAA49838 | 20 | 78 | 2 | 16351 |
| 20 | Post | 6 | Cystatin C precursor | NP_000090 | 7 | 39 | 1 | 16017 |
| 21 | Post | 6 | Cystatin SN precursor | NP_001889 | 48 | 285 | 4 | 16605 |
| 22 | Post | 6 | Cystatin SN precursor | NP_001889 | 36 | 170 | 3 | 16605 |
| 23 | Post | 6 | Keratin, type II cytoskeletal 1 | NP_006112 | 28 | 869 | 12 | 66170 |
| 24 | Post | 6 | Cystatin S precursor | NP_001890 | 25 | 127 | 2 | 16489 |
| 25 | Post | 6 | Cystatin SA precursor | NP_001313 | 23 | 143 | 2 | 16719 |
| 26 | Post | 6 | Keratin, type I cytoskeletal 9 | NP_000217 | 27 | 424 | 7 | 62255 |
The 15–17 kDa bands from saliva purified by SDS-PAGE of 6 pools of athletes´ prior to (Pre) or following (Post) maximal physical exertion. Note that Mass in last column refers to the protein identified as the origin of the peptide(s) analyzed, which were all collected near 15 kDa and trypsinized for analysis (Fig 2).
†Percent coverage of identified protein by peptide(s) analyzed.