Table 2.
Methylated metabolites LOD’s, LOQ’s, R2, and reproducibility
| Metabolite | LOD (pg/ml) | LOQ (pg/ml) | R2 | CV% | Ionization |
|---|---|---|---|---|---|
| Methyl chloroformate | 421 | 606 | 0.999 | 5.6 | Positive |
| Methyl phosphonic acid | 25 | 100 | 0.999 | 7.8 | Positive |
| Methyl-4-hydroxy benzoate | 159 | 390 | 0.999 | 6.3 | Positive |
| 1-methyl histdine | 1.0 | 5.0 | 0.999 | 8.9 | Positive |
| 5-methyl cytosine | 50 | 152 | 0.997 | 8.7 | Positive |
| 1-methyl nicotinamide | 32 | 56 | 0.993 | 9.3 | Positive |
| 2-amino-6-methyl mercaptopurine | 2.0 | 6.0 | 0.991 | 5.8 | Positive |
| 5-methyl cytidine | 6.0 | 16 | 0.999 | 9.5 | Positive |
| 5-deoxy-5-methyl thioadenosine | 2.0 | 6.0 | 0.999 | 7.5 | Positive |
| 5-methoxy-3-indole acetic acid | 66 | 119 | 0.999 | 5.3 | Negative |