Figure 1. Deletion of one Ptprb allele leads to increased TEK phosphorylation.
(A) Western blot of lung lysate from P5 Control, Tek+/-, PtprbNLS-LacZ/WT and Tek+/-;PtprbNLS-LacZ/WT mice revealed a 50% reduction in PTPRB expression in PtprbNLS-LacZ/WT (heterozygous) and Tek+/-;PtprbNLS-LacZ/WT mice. Likewise, TEK expression was reduced approximately 50% in Tek+/- and Tek+/-;PtprbNLS-LacZ/WT mice. (B) Immunoprecipitation of lung lysates from adult control and PtprbNLS-LacZ/WT using anti-TEK antibody followed by western blotting with anti-phospho tyrosine antibody revealed a marked elevation of TEK phosphorylation in PtprbNLS-LacZ/WT animals compared to littermate controls. Horizontal lines indicate population means. *p<0.05, ***p<0.001 as determined by Student’s t-test.
Figure 1—figure supplement 1. Uncropped images corresponding to the western blots presented in Figure 1A.
Figure 1—figure supplement 2. Uncropped images corresponding to the western blots presented in Figure 1B.
