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. 2019 Oct 17;8:e48474. doi: 10.7554/eLife.48474

Figure 3. Ptprb heterozygosity prevents ocular hypertension and RGC loss in Tek haploinsufficient mice.

(A) Elevated intraocular pressure (IOP) was observed in Tek+/- haploinsufficient mice at 30 weeks of age when measured by rebound tonography. As in Figure 2, this phenotype was prevented in Tek+/-;PtprbNLS-LacZ/WT double heterozygous animals, confirming the importance of TEK activation in IOP homeostasis (n = 6 WT, 14 PtprbNLS-LacZ/WT, 12 Tek+/- and 14 Tek+/-;PtprbNLS-LacZ/WT mice). (B) BRN3B staining in retinal flat-mounts from a second group of mice revealed loss of retinal ganglion cells by 19 weeks in Tek+/- mice. Littermate Tek+/-;PtprbNLS-LacZ/WT animals were protected, correlating with the reduced IOP observed (n = 4 WT, 4 PtprbNLS-LacZ/WT, 3 Tek+/- and 4 Tek+/-;PtprbNLS-LacZ/WT mice). Horizontal lines indicate population means. *p<0.05, ***p<0.001 as determined by 1-way ANOVA followed by Bonferroni’s correction.

Figure 3.

Figure 3—figure supplement 1. Retinal vasculature was normal in Tek and Ptprb haploinsufficient mice.

Figure 3—figure supplement 1.

(A) Confocal microscopy of P5 retinas stained with anti-CD31 antibody reveal normal progression of the superficial vasculature at both arterial and venous sprouting fronts. n = 6 (WT), 5 (Tek+/-), 3 (PtprbNLS-LacZ/WT), and 3 (Tek+/-;PtprbNLS-LacZ/WT). Horizontal lines indicate population means. Lack of significance was determined by 1-way ANOVA followed by Bonferroni’s correction. (B) Normal, fully developed retinal vasculature was observed in all three plexus layers in mice of all genotypes at P20. Scale bars indicate 250 μm in all panels.