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(A–B) ENMD-2076 suppressing the invasion ability of LN18, T98G cells in the Transwell assay. 5 random fields were counted for each condition. (C) Protein levels of E-cadherin, Vimentin, Cytokeratin and MMP-9 evaluated by immunofluorescence demonstrated ENMD-2076 inhibit glioblastoma invasion by suppressing EMT. (D–F) Flow cytometry results showed that ENMD-2076 induced glioblastoma cells (LN18, T98G) apoptosis. ENMD-2076 showed pro-apoptotic effects on LN18, T98G cells.
