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NLRP3 overexpression induces IL-1β secretion and cellular senescence. (A) The efficiency of NLRP3 overexpression was assayed with qPCR and western blot. (B) Senescent cells were counted and visualized in graphs (40 × magnification). The levels of p53, p21, p16, and IL-1β were measured by qPCR and western blot. Data are expressed as mean ± SD from three experiments. *P<0.05, **P<0.01, and ***P<0.001.
