Video 2. Dissociation pathway of the UBC13/TRAF6^RING native complex observed by steered MD.

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The movie shows the order of contact disruption during enforced dissociation of TRAF6^RING from UBC13 (Figure 5A–C, Figure 5—figure supplement 1A). The loss of S96-P106 and hydrophobic interactions occur by ~9 ns. The R14-E69 salt-bridge persists until ~ 15 ns following which, complete dissociation occurs. Residues R6/R14/S96 in UBC13 (Green) and D57/E69/P106 in TRAF6^RING (Blue) are shown in stick representation.