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. 2019 Nov 22;13(6):064119. doi: 10.1063/1.5123476

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Copyright © 2019 Author(s)

Published under license by AIP Publishing.

1932-1058/2019/13(6)/064119/13/$30.00

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FIG. 2. — BMECs cultured in the device remain viable and form a confluent monolayer in devices with a hydrogel. (a)–(c) The phase contrast image (a) and calcein AM staining [(b) and (c)] of BMECs in devices with a 0.4 μm membrane. (d)–(f) The phase contrast image (d) and calcein AM staining [(e) and (f)] of BMECs in devices with an 8.0 μm membrane. All images show full coverage of the membrane with live cells. Phase contrast imaging was performed 1 day after seeding, and live cell staining was performed 2 days after seeding. Scale bars for (a), (b), (d), and (e) indicate 400 μm, and scale bars for (c) and (f) indicate 1000 μm.