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BMECs seeded in devices with a hydrogel show proper tight protein expression. Immunostaining of the BMEC layer on a 0.4 μm (a)–(c) or 8 μm (d)–(f) membrane for ZO-1 [(a) and (d)], occludin [(b) and (e)], and claudin-5 [(c) and (f)]. Scale bars indicate 200 μm.
