Skip to main content
. 2019 Nov 22;13(6):064119. doi: 10.1063/1.5123476

FIG. 7.

FIG. 7.

Studying changes in barrier properties and junctional protein expression of BMECs and activation of astrocytes after TGF-β1 treatment. (a) The effect of TGF-β1 on the formation of stress fibers and junctional protein expression. White arrows show frayed junctions in ZO-1 staining. (b) The effect of TGF-β1 on TEER of the BMEC layer for devices with only BMECs (monoculture) and coculture of BMECs and astrocytes (* indicates P < 0.05 compared to control). (c)–(e) Astrocytes cultured in an untreated device fixed and stained with WGA (c) and for GFAP (d). (e) The 3D image of the astrocytes stained for GFAP (green) and cell membrane (WGA; red) in an untreated device. (f)–(h) Astrocytes cultured in devices treated with TGF-β1 in the apical channel fixed and stained with WGA (f) and for GFAP (g). (h) The 3D image of astrocytes stained for GFAP (green) and cell membrane (red) in a treated device showing upregulation of GFAP expression in the astrocytes. Scale bars indicate 200 μm.