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. 2019 Nov 22;9:17427. doi: 10.1038/s41598-019-54036-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Adipocytes within the developing skeleton express adiponectin, but not UCP1, by lineage tracing. (a) Representative images of Adipoq^Cre+/mTmG+ and UCP1^Cre+/mTmG+ inguinal white adipose tissue (iWAT), constitutive bone marrow adipose tissue (cBMAT, caudal vertebrae), and regulated bone marrow adipose tissue (rBMAT, tibia). The presence of green (GFP-positive) cells indicates expression of Cre and conversion of the mTmG reporter. White boxes in images show inset for images to the right (GFP/RFP). Far right images (perilipin) demonstrate that the adipocytes in the GFP/RFP images are perilipin-positive. Images were acquired at 10X, scale bar is 50 µm or 25 µm for insets. (b–d) Quantification of Adipoq-Cre or Ucp1-Cre-mediated GFP expression in adipocytes relative to total number of adipocytes. White bars are the percent of adipocytes expressing GFP. Grey bars are the percent of adipocytes that do not express GFP (instead express RFP). Tissues collected from 3-week-old male and female mice (b) 16-week-old males (C) or females (d) treated with saline (−) or 0.3 mg/kg CL316,243 (CL) for 6-days. (b–d) Mean ± S.D. Sample sizes: (b) n = 5–6 (c) n = 3–5, (d) n = 3–7.