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. 2019 Nov 22;9:17368. doi: 10.1038/s41598-019-53415-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Aβ-GFP Tg mice do not exhibit the extracellular deposition of Aβ but increased phosphorylated tau. (A,B) Anti-Aβ (6E10) immunostaining of brain sections of 24-month-old non-Tg (A) and Aβ-GFP Tg mice (B). Insets in (A) and (B) show the magnification of the boxed areas of the hippocampal CA1 pyramidal cell layer. Although the pyramidal cells of hippocampus CA1 regions were immunopositive in Aβ-GFP Tg mice (inset in B), no extracellular amyloid depositions were observed in any regions we examined. No immunoreactivity was observed in non-Tg littermates (A). Scale bars; 500 μm (A,B), 250 μm (insets). (C) Representative immunoblots showing the expression of phosphorylated tau (p-tau) in hippocampal homogenates (3- and 18-month-old: 30 μg/lane each) in non-Tg (lanes 1, 3) and Aβ-GFP Tg (lanes 2, 4). Immunoblotting of actin served as loading control and band intensities were normalized by it. Histograms represent the ratios of phosphorylated tau in Aβ-GFP Tg against non-Tg mice. No significant difference was observed between Aβ-GFP Tg and non-Tg mice in young age but tau phosphorylation was increased significantly in Aβ-GFP Tg mice at 18-month-old (*p < 0.05, Mann-Whitney’s U test, n = 8 (3 M) and 8 (18 M) animals each, data are presented as means ± SEM).