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. 2019 Nov 22;9:17368. doi: 10.1038/s41598-019-53415-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Aβ-GFP fusion protein did not induce neuronal cell death in hippocampus. (A,C) Anti-NeuN immunostaining of coronal sections of Aβ-GFP Tg and non-Tg littermates at 3- month (A) and 18- month (C) old. (B,D) Total number of NeuN-positive cells in the CA1-CA2 region of hippocampus measured using Stereo Investigator. Histograms show that there were no significant differences in the numbers of NeuN-positive cells between Aβ-GFP Tg and non-Tg in either 3-month (B) or 18-month (D) old mice (Mann-Whitney’s U test, n = 7 animals each, data are presented as means ± SEM). Scale bars; 500 μm.