Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 22;9:17400. doi: 10.1038/s41598-019-53726-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Target genomic locus and integrative expression vectors. (a) Schematic of B. theta genomic locus PUL75 containing genes required for homogalacturonan (HG) utilization (top). These genes are deleted in the B. theta ΔPUL75 strain (bottom). (b) Growth profiles of wild-type (WT) B. theta (grey) and the ΔPUL75 strain (purple) in MM containing either glucose (GLC) or HG as sole carbon source. Growth was measured by OD₆₀₀ readings over the course of 16 h. Values represent the average of three biological replicates. Variation is represented by standard error of the mean (s.e.m.) (c) Plasmid maps of the pINT (left) and pNBU2 (right) vectors. The orientation of the DX, AG, and ON, expression cassettes and the sequence of the MCS are indicated.