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. 2019 Nov 22;9:17400. doi: 10.1038/s41598-019-53726-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inducible expression cassettes generate a dose dependent response in NanoLuc activity in B. theta. (a) Schematic of wild-type regulation by PUL48. P_DX is activated by the SusR-like protein SusR^DX in the presence of DX. (b) Schematic of wild-type AG regulation. P_AG from PUL5 is activated by the HTCS protein HTCS^AG in response to the presence of AG. Schematic representations of monomeric glucose (GLC; blue circle) the polymer dextran (DX) composed of a chain of GLC; and arabinogalactan (AG), composed of a galactose (GAL, yellow circle) backbone and arabinose side chains (green star). (c) NanoLuc activity under constitutive expression by the P_ON promoter is measured post induction with GLC (black bar), DX (blue bar), and AG(yellow bar). Luminescence signal for NanoLuc production under the (d) DX or (e) AG inducible promoters in the presence of increasing concentrations of DX or AG, respectively. Values in (d–f) represent the average and s.e.m. for three biological replicates. Asterisks represent levels of significance (*p < 0.05, **p < 0.01, ***p < 0.001).