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. 2019 Nov 22;9:17400. doi: 10.1038/s41598-019-53726-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Episomal transgene expression results in higher enzyme activity when compared to chromosomal-based expression. (a) Vector map of the extra-chromosomal expression pEP_DX vector. (b) Luminescence signal detected from wild-type (WT), P_ONSusR^DX, P_DXSusR^DX, P_AGSusR^DX, and P_DXHTCS^AG + P_AGSusR^DX strains containing the NanoLuc under the control of the episomal pEP_DX promotor, in the presence of GLC (black bars), DX (blue bars), AG (yellow bars) or the DX and AG mixture (green bars). (c) Comparison of luminescence from engineered regulatory strains when NanoLuc is encoded in a plasmid or in the chromosome. Error bars represent s.e.m. for three biological replicates. Asterisks represent levels of significance (*p < 0.05, **p < 0.01, ***p < 0.001).