Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 22;9:17374. doi: 10.1038/s41598-019-53574-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Alpha-SNAP localization during meiotic maturation in wild type and hyh oocytes. (a) Alpha-SNAP was immunodetected at two different stages during meiotic maturation: Germinal vesicle-intact oocytes (GV) and Metaphase II oocytes (MII). Green indicates positive staining for primary alpha-SNAP antibody detected by a secondary antibody conjugated with DyLight 488; DIC shows differential interference contrast (DIC) images. The right column shows the fluorescence intensity profiles for alpha-SNAP. Fluorescence intensities were measured along dashed yellow lines traced in each oocyte. The intensity of alpha- SNAP is indicated by green lines. Scale bar: 20 μm. (b) Cortical and cytoplasmic patterns of protein distribution. The cortical region was defined as the region of 10 μm thickness from the oocyte plasma membrane towards the oocyte centre. Those cells in which fluorescence decay at 10 μm were considered to present a cortical staining, and those cells which present high fluorescence at 10 μm towards the center and beyond were considered to present cytoplasmic staining. Percentage analysis was assessed. Number of oocytes: GV wild type = 33; GV hyh = 34; MII wild type = 27; MII hyh = 12. (c) Histogram showing fluorescence intensity quantification (A.U.: arbitrary units) for wild type GV and MII oocytes compared to hyh ones.