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. 2019 Nov 22;9:17374. doi: 10.1038/s41598-019-53574-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NSF inmunodetection during meiotic maturation in wild type and hyh MII oocytes. (a) NSF was detected by indirect immunoflurescence in wild type and mutant homozygous (hyh) GV-intact oocytes (GV) and MII oocytes (MII). Red indicates positive staining for primary NSF antibody detected by a secondary antibody conjugated to Alexa Fluor 594; blue indicates DNA, labeled with Hoechst 3342; DIC shows differential interference contrast (DIC) images. Right column in each panel shows the fluorescence intensity profiles for NSF. Fluorescence intensities were measured along equatorial dashed yellow lines traced in each oocyte. The intensity of NSF is indicated by red lines. Scale bar: 20 μm. (b) Histogram showing fluorescence intensity quantification (A.U.: arbitrary units) for wild type GV and MII oocytes compared to hyh ones. ***p ≤ 0.001 (Student’s t-test).