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. 2019 Nov 22;9:17374. doi: 10.1038/s41598-019-53574-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cortical granules at the ultrastructural level in wild type and mutant hyh MII oocytes. (a) Representative transmission electron microscopy images of the cortex in MII oocytes (MII) in wild type and mutant homozygous (hyh) mice.Cortical granules (arrow heads) are linearly arranged below the plasma membrane. Note microvillous processes of oolema. zp: zona pellucid; pvs: perivitelline space; pm: plasma membrane; cpl: cytoplasmic lattice. Scale bar: 1 μm. Frequency histograms were plotted for cortical granules size (major axis) (b) and distance to the plasma membrane (c). Values on the x-axis represent bins of size (a) or distance (b) data. The number of analyzed cortical granules (CG) and images are the same as in Table 2. Data were compared using the Kolmogorov-Smirnov test for two sets of data. Individual size and CG distances in wild type and hyh oocytes show similar distribution profiles (N.S. p > 0.05).