. 2019 Nov 22;9:17374. doi: 10.1038/s41598-019-53574-8

Table 2.

Ultrastructural analysis of mitochondria in wild type and mutant homozygous (hyh) MII oocytes.

Genotype	Vacuolated mitochondria (%)	Mitochondria density (/µm2)	Mean mitochondria size (µm)	Mean vacuole size (µm)
wild type	43,58 ± 1,47 (1745)	0,485 ± 0,040 (48)	0,572 ± 0,007 (501)	0,380 ± 0,010 (289)
hyh	49,50 ± 1,39** (2115)	0,488 ± 0,037 N.S (58)	0,503 ± 0,006*** (524)	0,287 ± 0,007*** (257)

Genotype

Vacuolated mitochondria (%)

Mitochondria density (/µm2)

Mean mitochondria size (µm)

Mean vacuole size (µm)

wild type

43,58 ± 1,47

(1745)

0,485 ± 0,040

(48)

0,572 ± 0,007

(501)

0,380 ± 0,010

(289)

hyh

49,50 ± 1,39** (2115)

0,488 ± 0,037 N.S (58)

0,503 ± 0,006*** (524)

0,287 ± 0,007*** (257)

Percentage of vacuolated mitochondria, mitochondria density, mitochondria and mitochondrial vacuole size (major axis) were evaluated. For mitochondria density analysis, the total number of mitochondria in single TEM sections were counted, and based on the scale bar, the number of mitocondria per unit area (µm²) was calculated. Numbers in parenthesis indicate the number of analyzed mitochondria or analyzed sections in the case of mitochondria density. Data are shown as mean ± SEM.***p ≤ 0.001, **p ≤ 0.01, N.S. p > 0,05 (Student’s t-test).