Table 4.

Oocyte roundness analysis in MII oocytes of wild type, heterozygous and mutant homozygous (hyh) mice.

Genotype	oocyte N°	Roundness
wild type	43	0,980 ± 0,002
heterozygous	29	0,978 ± 0,001
hyh(typical CG distribution)	30	0,976 ± 0,002
hyh(atypical CG distribution)	9	0,905 ± 0,018***

ImageJ’s Roundness parameter calculation was measured using differential interference contrast (DIC) images from MII oocytes of different genotypes. Roundness resulted significantly lower in hyh MII oocytes with atypical CG distribution than in those oocytes with typical CG distribution (wild type, heterozygous and mutant homozygous (hyh); ***p ≤ 0.001; Tukey’s test for multiple comparisons).