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. 2019 Nov 22;9:17365. doi: 10.1038/s41598-019-53907-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Disabled spheroid and organoid formation by FGFR-inhibition in patient-derived CRC cells. (A) Surgical biopsies were enzymatically digested to obtain a suspension of single cells. The naïve unsorted cells were cultured in FGF2 containing CSC-medium or with the FGFR-inhibitor SU-5402. (Parts of the scheme were created in the Mind the Graph platform, www.mindthegraph.com (2019), adaptations were made, Illustrations were made under a creative commons license (https://creativecommons.org/licenses/by-sa/4.0/) (B) Cell culture initiation of naïve unsorted bulk cells (p0) after tissue disintegration, cultured in CSC-media (CSCM) or with FGFR-inhibitor (SU-5402) under low-attachment conditions as floating spheres or (C) as Matrigel-embedded organoids.