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. 2019 Nov 22;6:277. doi: 10.1038/s41597-019-0292-2

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© The Author(s) 2019

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Fig. 3 — Calibration of optimally-positioned particles. Optical properties of optimally-positioned calibration beads show a linear relationship between the forward scatter and the position-sensitive detectors (D1) normalized to 1-μm calibration beads, which is represented by the two linear regression models (red lines). Grey lines represent the 95% confidence interval of the two regression models.