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. 2019 Nov 22;2:427. doi: 10.1038/s42003-019-0671-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Drosophila larval brain qNSC protrusions contain clustered mitochondria as observed by TEM. a Drosophila larval brain. Larva, left; brain, right (schematic diagrams). Neuropil, gray regions in the brain lobe (BL) and thoracic ventral nerve cord (tVNC). b–h TEM images, amino-acid-depleted conditions. b Larval brain, low magnification. N, neuropil. c BL region (inset, gray box) at higher magnification in d and e, showing qNSCs (e, yellow overlays). f tVNC with mitochondria (M) in protrusions (P). g qNSC protrusions form junctions with the neuropil (magenta arrowheads); small mitochondria (cyan arrows) in the neuropil near a qNSC protrusion end. Putative glial cell (upper right) with an irregular cell body. Abn M, abnormal mitochondria (see Supplementary Fig. 1). h Clustered mitochondria in a qNSC protrusion neck (center). NB, neuroblast; Nu, nucleolus. Bars: 10 µm (b); 2 µm (c); 500 nm (d–h).