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. 2019 Nov 22;2:432. doi: 10.1038/s42003-019-0676-z

Fig. 3.

Fig. 3

The TonB box in DdvT is important for DDVA uptake. a Growth of TonB box mutant cells on DDVA. Cells of SYK-6(pSEVA338 [vector]), ∆ddvT(pSEVA338), ∆ddvT(pS-ddvT), ∆ddvT(pS-ddvTV42A), ∆ddvT(pS-ddvTT43A) and ∆ddvT(pS-ddvTV42A-T43A) were cultured in Wx medium containing 5 mM DDVA. b DDVA conversion by resting cells of TonB box mutants. Cells of SYK-6(pSEVA338), ∆ddvT(pSEVA338), ∆ddvT(pS-ddvT), ∆ddvT(pS-ddvTV42A), ∆ddvT(pS-ddvTT43A) and ∆ddvT(pS-ddvTV42A–T43A) were incubated with 100 µM DDVA. c DDVA uptake by TonB box mutant cells. The β-galactosidase activities of cells of SYK-6(pSEVA338 + pS-XR), ∆ddvT(pSEVA338 + pS-XR), ∆ddvT(pS-ddvT + pS-XR), ∆ddvT(pS-ddvTV42A + pS-XR), ∆ddvT(pS-ddvTT43A + pS-XR) and ∆ddvT(pS-ddvTV42A–T43A + pS-XR) incubated in Wx-SEMP with or without 100 µM DDVA were measured. Each value is the average ± the standard deviation of n = 3 independent experiments. ns, P > 0.05, *P < 0.05, ***P < 0.001, ****P < 0.0001 (one-way ANOVA with Dunnett’s multiple comparisons post-test).