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. 2019 Nov 22;9:17417. doi: 10.1038/s41598-019-53870-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of ROX plus siVEGF/siVEGFR on EC proliferation and migration. (a) BrdU staining of ECs treated with the indicated reagents for 24 h (×200 magnification). Representative images are shown. (b) The number of BrdU-positive cells presented as the mean ± SEM of three independent experiments. (c) Rat EC monolayers were wounded with a pipette and treated with the indicated reagents for 24 h. Representative images (×100 magnification) are shown. (d) The distance the cells had migrated. The results are the mean ± SEM of three independent experiments. ***P < 0.001 vs. PBS control; ^&&&P < 0.001 vs. 1.0 μM ROX.