Table 2.

Further investigation of pH and DNA–PEI concentration for CHIKV VLP transient transfection by a DoE study in the ambr® 15 bioreactor

Vessel	pH	Inoculum source	DNA (mg/L)– PEI (mg/L)
1	7.2 ± 0.1	Middle exponential	20–40
2			30–40
3			40–40
4			20–20
5			30–20
6			40–20
7	6.8 ± 0.1	Middle exponential	20–40
8			30–40
9			40–40
10			20–20
11			30–20
12			40–20

pH controls were not overlapped in this study. Vessel 1 (DNA 20 mg/L–PEI 40 mg/L) was used as a control