Table 5.

Effects of sitagliptin in SCE and PI in cultured human lymphocytes

Test substance (µg/ml)	Concentartion (µg/ml)	Period	S9 mix	M1	M2	M3	Min–max SCE	Total SCE	SCE/cell ± SE	PI ± SE
DMSO	1	24 h	–	20	137	243	159–194	698	174.5 ± 7.31	2.56 ± 0.06
MMC	0.16	24 h	–	28	256	116	975–1429	4735	1183.8 ± 111.69	2.22 ± 0.03
Vildagliptin	125	24 h	–	38	122	250	148–201	694	173.5 ± 12.55	2.58 ± 0.04
Vildagliptin	250	24 h	–	15	100	285	145–208	656	164 ± 14.75	2.68 ± 0.02
Vildagliptin	500	24 h	–	16	118	266	119–215	615	153.75 ± 13.42	2.63 ± 0.04
DMSO	1	48 h	–	42	143	215	119–215	699	174.75 ± 20.27	2.43 ± 0.06
MMC	0.16	48 h	–	108	290	2	2380–4049	11988	2997 ± 380.60	1.74 ± 0.13
Vildagliptin	125	48 h	–	24	149	227	160–199	703	175.75 ± 9.20	2.51 ± 0.06
Vildagliptin	250	48 h	–	18	162	220	141–237	720	180 ± 20.47	2.51 ± 0.01
Vildagliptin	500	48 h	–	27	156	217	93–243	752	188 ± 33.54	2.48 ± 0.06
DMSO	1	S9 mix	+	44	324	32	115–174	556	139 ± 14.16	1.97 ± 0.02
CP	45	S9 mix	+	100	297	3	532–628	2310	577.5 ± 22.14	1.76 ± 0.02
Vildagliptin	125	S9 mix	+	49	328	23	136–188	636	159 ± 12.38	1.94 ± 0.02
Vildagliptin	250	S9 mix	+	51	327	22	126–185	587	146.75 ± 13.09	1.93 ± 0.02
Vildagliptin	500	S9 mix	+	45	333	22	127–181	626	156.5 ± 11.71	1.94 ± 0.02
DMSO	1	24 h	–	35	152	213	83–225	646	161.50 ± 35.72	2.45 ± 0.10
MMC	0.16	24 h	–	105	229	66	857–1252	4264	1073.5086.87	1.90 ± 0.16
Sitagliptin	250	24 h	–	63	176	161	121–224	631	157.75 ± 22.98	2.25 ± 0.19
Sitagliptin	500	24 h	–	62	151	187	94–231	717	179.25 ± 29.73	2.31 ± 0.12
Sitagliptin	1000	24 h	–	59	167	174	76–270	678	169.50 ± 40.75	2.29 ± 0.12
DMSO	1	48 h	–	53	150	197	101–267	716	179.00 ± 39.66	2.36 ± 0.19
MMC	0.16	48 h	–	129	263	8	1749–3751	10570	2642.50 ± 413.59	1.70 ± 0.13
Sitagliptin	250	48 h	–	63	212	128	117–230	732	183.00 ± 26.23	2.18 ± 0.13
Sitagliptin	500	48 h	–	57	240	103	91–207	628	157.00 ± 25.34	2.12 ± 0.03
Sitagliptin	1000	48 h	–	173	206	21	203–299	1036	259.00 ± 22.57	1.62 ± 0.08*
DMSO	1	S9 mix	+	59	310	31	114–174	528	132.00 ± 14.17	1.93 ± 0.07
CP	45	S9 mix	+	72	325	3	500–676	2391	597.75 ± 39.46	1.83 ± 0.05
Sitagliptin	250	S9 mix	+	44	334	22	127–216	632	158.00 ± 20.32	1.95 ± 0.04
Sitagliptin	500	S9 mix	+	39	348	13	138–168	626	156.50 ± 7.23	1.94 ± 0.03
Sitagliptin	1000	S9 mix	+	82	293	25	115–189	628	157.00 ± 15.81	1.86 ± 0.05

* Significantly different from the solvent control p < 0.05 (ANOVA)