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. 2019 Nov 22;2:428. doi: 10.1038/s42003-019-0678-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Sampling framework for B. pseudomallei isolates from the case control study. a The chart shows the number of clinical and environmental isolates from patients and/or household water supplies of cases (patients with melioidosis) and controls (patients with non-infectious conditions admitted during the same period). b Temporal distribution of environmental and disease isolates in the discovery dataset collected from June 2010 to January 2012. With the exception of months with no house visits, the number of monthly clinical and environmental samples collected were positively correlated (linear regression, adjusted R-square = 0.259, p value = 0.026). c Spatial and temporal distribution of environmental and disease isolates in the validation dataset from the public database.