Table 1.
Nr. | Primer sequences | PCR product, bp | Comments |
---|---|---|---|
1 |
Forward: 5′-ACAGCGAAAGTATCATTCATC-3′ Reverse: 5′-AACTGATAGAGGCTGTTTGG-3′ |
1190 | Positive if construct has been inserted in chromosome 5 from position ~91496672→ |
2 |
Forward: 5′-ATCTTGGCTCACTGCAATC3′ Reverse: 5′-CTTCTCTCTAGTGTGCAAAGC-3′ |
849 | Positive if construct has been inserted in chromosome 5 from position ← 91514836 ~ |
3 |
Forward: 5′-GTGTCACTTCTTAGCATAACG-3′ Reverse: 5′-CTTCTCTCTAGTGTGCAAAGC-3′ |
480 | Positive if chromosome 5 as in original reference |
4 |
Forward: 5′-ATCTTGGCTCACTGCAATC-3′ Reverse: 5′-AACTGATAGAGGCTGTTTGG-3′ |
1007 | Positive if double construct of TH promoter and SNCA exists |