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. 2019 Nov 22;9:17382. doi: 10.1038/s41598-019-54034-z

Table 1.

Primer pairs and PCR product information for TH-SNCA*A30P*A53T genotyping.

Nr. Primer sequences PCR product, bp Comments
1

Forward:

5′-ACAGCGAAAGTATCATTCATC-3′

Reverse:

5′-AACTGATAGAGGCTGTTTGG-3′

1190 Positive if construct has been inserted in chromosome 5 from position ~91496672→
2

Forward:

5′-ATCTTGGCTCACTGCAATC3′

Reverse:

5′-CTTCTCTCTAGTGTGCAAAGC-3′

849 Positive if construct has been inserted in chromosome 5 from position ← 91514836 ~
3

Forward:

5′-GTGTCACTTCTTAGCATAACG-3′

Reverse:

5′-CTTCTCTCTAGTGTGCAAAGC-3′

480 Positive if chromosome 5 as in original reference
4

Forward:

5′-ATCTTGGCTCACTGCAATC-3′

Reverse:

5′-AACTGATAGAGGCTGTTTGG-3′

1007 Positive if double construct of TH promoter and SNCA exists