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. 2019 Nov 22;9:17382. doi: 10.1038/s41598-019-54034-z

Table 2.

Cycling parameters used to genotype TH-SNCA*A30P*A53T mice.

Step Temperature (°C) Time (min)
1. Initial denaturation 94 °C 3 min
2. Denaturation 94 °C 0:30 min
3. Annealing 60 °C, −0,3 °C per cycle 0:30 min
4. Extension 72 °C 1:30 min
Go to step 2 33x times
5. Final extension 72 °C 10 min
6. Hold 4 °C