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. 2019 Nov 22;9:17428. doi: 10.1038/s41598-019-54022-3

Figure 2.

Figure 2

Comparative anatomies of the apple fruit tissue and callose presence at the beginning (A,B) and after 16 days of fungal infection (C,D). Images of equivalent control samples in experiment I (EH). Sections of apple tissue after injection with addition of the β-GlcY on the onset day (I and J) and on day 16 of the experiment (K,L). Control samples in experiment II (M–P). Semi-thin sections stained with Toluidine blue (A,E,I,M,C,G,K,O) and with Aniline blue to detect callose (B,F,J,N,D,H,L,P). CLSM. Abbreviations: cu – cuticle; ep – epidermal layer; h – hypodermal layer; p – parenchyma. Bars 50 µm.