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. 2019 Nov 22;9:17428. doi: 10.1038/s41598-019-54022-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in AGP immunolocalization recognized by the LM2 antibody in apple tissue at different time points (0, 4, 8, 16 days) of infection by P. spinulosum (A–D) and in equivalent control samples (E–H). Immunolocalization of the LM2 epitope in tissue treated by inoculum with the β-GlcY on the inoculation day (I) and on day 16 after the inoculation (J). Control samples (K,L). Magnification of infected parts of fruit after 16 days of experiment II (M–O). CLSM. Immunolabelling reaction with LM2 mAb and Calcofluor White. Bars 100 µm.